Brilliant Green：a long-buried water-soluble AIEgen with near-infrared fluorescence for living cell actin staining

Luxuan Liu; Yayun Wu; Dongbao Tang; Zhaoyuan Jia; Jiaoli Wang; Ping Gong; Xiaohong Fang; Yuyu Tan; Pengfei Zhang

doi:10.1117/12.3036694

13 July 2024 Brilliant Green：a long-buried water-soluble AIEgen with near-infrared fluorescence for living cell actin staining

Luxuan Liu, Yayun Wu, Dongbao Tang, Zhaoyuan Jia, Jiaoli Wang, Ping Gong, Xiaohong Fang, Yuyu Tan, Pengfei Zhang

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Proceedings Volume 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024); 132080K (2024) https://doi.org/10.1117/12.3036694
Event: 3rd International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 2024, Nanchang, China

Abstract

Observation of actin in cells is crucial for the investigation of cell motility, protein transcription, pathogen infection, and cell cycle research. In this study, we have identified Brilliant Green (BG) as a water-soluble AIEgen (luminogen with aggregation-induced emission properties) that can be utilized for actin staining. Our experimental findings highlight the effectiveness of BG for long-term live cell imaging, as well as its remarkable stability and resistance to photobleaching.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Luxuan Liu, Yayun Wu, Dongbao Tang, Zhaoyuan Jia, Jiaoli Wang, Ping Gong, Xiaohong Fang, Yuyu Tan, and Pengfei Zhang "Brilliant Green：a long-buried water-soluble AIEgen with near-infrared fluorescence for living cell actin staining", Proc. SPIE 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 132080K (13 July 2024); https://doi.org/10.1117/12.3036694

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KEYWORDS

Fluorescence

Cytoskeletons

Dyes

Water

Visualization

Antibodies

Confocal microscopy

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