High-resolution imaging using endoscopic holography

Hans I. Bjelkhagen

doi:10.1117/12.22360

1 August 1990 High-resolution imaging using endoscopic holography

Hans I. Bjelkhagen

Proceedings Volume 1321, Modern Technologies Applied to Medical Practice; (1990) https://doi.org/10.1117/12.22360
Event: Modern Technologies Applied to Medical Practice, 1989, Berlin, Germany

Abstract

Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help to their control. 1.

Citation Download Citation

Hans I. Bjelkhagen "High-resolution imaging using endoscopic holography", Proc. SPIE 1321, Modern Technologies Applied to Medical Practice, (1 August 1990); https://doi.org/10.1117/12.22360

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