The structure of lepidopteran wing scales plays an important role in the regulation of their biological behaviors, making the 3D imaging of them very useful. However, most of the current three-dimensional microscopic methods are usually destructive to the samples. Fourier light-field microscopy has the characteristics of real-time imaging and multi-angle visualization, thus becomes a preferred method to study the three-dimensional structure of structural color scales in lepidopteran insects. In this study, Fourier light-field microscopy was used to compare the imaging of Morpho helenor and Chrysiridia rhipheus under 10X and 50X objective lenses, where the results demonstrated the difference structure of the wing scale between these two species and the corresponding view angle dependent color variations. This study demonstrates the unique advantages and application prospects of Fourier light-field microscopy for 3D imaging of the large-field thick samples
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