Reduced defocus degradation in a system for high-speed three-dimensional digital microscopy

Laura A. Quesenberry; V. Arlene Morris; Ian R. Neering; Stuart R. Taylor

doi:10.1117/12.44140

1 May 1991 Reduced defocus degradation in a system for high-speed three-dimensional digital microscopy

Laura A. Quesenberry, V. Arlene Morris, Ian R. Neering, Stuart R. Taylor

Proceedings Volume 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences; (1991) https://doi.org/10.1117/12.44140
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Most low-light-level 3D bio-imaging systems use sensors with high spatial resolution but relatively low temporal resolution. The authors aim to measure events in skeletal muscle cells that include (1) changes in cross-sectional area during contraction and (2) regions of contracting cells illuminated by Ca²⁺-activated photoproteins compared with those that are dark or partly lit. This paper outlines some limits of the system's ability to detect these events.

Citation Download Citation

Laura A. Quesenberry, V. Arlene Morris, Ian R. Neering, and Stuart R. Taylor "Reduced defocus degradation in a system for high-speed three-dimensional digital microscopy", Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); https://doi.org/10.1117/12.44140

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