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A quantitative technique based on fluorescence microscopic detection has been developed for measurements of photosensitizer distributions in sections of tissue and cultured cells. Imaging of photosensitizer fluorescence was achieved with negligible interference from sensitizer photodegradation and tissue autofluorescence using laser excitation and detection with a highly sensitive CCD (charge-coupled device) camera system. Techniques for the measurement of sensitizer fluorescence decay parameters in living biological cells are also described.
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Alexander J. MacRobert, David Phillips, "Fluorescence imaging in photodynamic therapy," Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); https://doi.org/10.1117/12.50461