Selection of Norway spruce somatic embryos by computer vision

Jari J. Hamalainen; Kari J. Jokinen

doi:10.1117/12.144028

12 May 1993 Selection of Norway spruce somatic embryos by computer vision

Jari J. Hamalainen, Kari J. Jokinen

Proceedings Volume 1836, Optics in Agriculture and Forestry; (1993) https://doi.org/10.1117/12.144028
Event: Applications in Optical Science and Engineering, 1992, Boston, MA, United States

Abstract

A computer vision system was developed for the classification of plant somatic embryos. The embryos are in a Petri dish that is transferred with constant speed and they are recognized as they pass a line scan camera. A classification algorithm needs to be installed for every plant species. This paper describes an algorithm for the recognition of Norway spruce (Picea abies) embryos. A short review of conifer micropropagation by somatic embryogenesis is also given. The recognition algorithm is based on features calculated from the boundary of the object. Only part of the boundary corresponding to the developing cotyledons (2 - 15) and the straight sides of the embryo are used for recognition. An index of the length of the cotyledons describes the developmental stage of the embryo. The testing set for classifier performance consisted of 118 embryos and 478 nonembryos. With the classification tolerances chosen 69% of the objects classified as embryos by a human classifier were selected and 31$% rejected. Less than 1% of the nonembryos were classified as embryos. The basic features developed can probably be easily adapted for the recognition of other conifer somatic embryos.

Citation Download Citation

Jari J. Hamalainen and Kari J. Jokinen "Selection of Norway spruce somatic embryos by computer vision", Proc. SPIE 1836, Optics in Agriculture and Forestry, (12 May 1993); https://doi.org/10.1117/12.144028

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