Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study

Stefano Beretta; Giuseppe Chirico; Giancarlo Baldini; U. Kapp; G. Badaracco

doi:10.1117/12.146215

17 June 1993 Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study

Stefano Beretta, Giuseppe Chirico, Giancarlo Baldini, U. Kapp, G. Badaracco

Author Affiliations +

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146215
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The effect of the insertion of different amounts (from 0 to 6) of the curved sequence AluI in pUC18m plasmid (2686 base pairs, bp) is studied by dynamic light scattering. This sequence is a highly repeated 113 base pairs long sequence from Artemia Franciscana shrimp. A 30% compaction of the plasmids containing 2 and 6 adjacent AluI sequences compared to pUC8 plasmid (2717 bp) is observed. Furthermore the behavior of the translational diffusion coefficient D_t versus the number of adjacent AluI insertion is not monotonic.

Citation Download Citation

Stefano Beretta, Giuseppe Chirico, Giancarlo Baldini, U. Kapp, and G. Badaracco "Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146215

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