Computerized morphodensitometric analysis of blood cells from individuals exposed to low-dose radiation

Victor F. Kopylov; Alexander V. Zhukotsky; Emmanuil M. Kogan; Tatiana G. Putina; Oleg V. Marchenko; Elena B. Lomakina; Sergei A. Chernysh

doi:10.1117/12.180785

21 July 1994 Computerized morphodensitometric analysis of blood cells from individuals exposed to low-dose radiation

Victor F. Kopylov, Alexander V. Zhukotsky, Emmanuil M. Kogan, Tatiana G. Putina, Oleg V. Marchenko, Elena B. Lomakina, Sergei A. Chernysh

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180785
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

This study was carried out as part of the state program Children Affected by the Chernobyl Accident and used materials and results of the medical examinations of children living in radio-contaminated areas of the Bryansk region. The health status of children and adolescents exposed to low-dose radiation is causing increasing concern. Our attention was focused on assessing chromatin disorders in interphase lymphocyte nuclei and determining diagnostic criteria for lymphocytes and erythrocytes through measurement of their morphodensitometric parameters using a system of our own design called DIAMORF. Blood cells from children and adolescents exposed to low-dose radiation were examined after processing images of DNA- stained lymphocytes and unstained erythrocytes in a computer analyzer. The new method was used to study the fine supromolecular structure of the chromatin network. An optoelectronic image analyzer can differentiate between different areas of the erythrocytes surface and determine their curvatures. Parameters of cellular changes in response to low-dose radiation were evaluated quantitatively by measuring the optical density index and a number of other optical and geometric parameters.

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Victor F. Kopylov, Alexander V. Zhukotsky, Emmanuil M. Kogan, Tatiana G. Putina, Oleg V. Marchenko, Elena B. Lomakina, and Sergei A. Chernysh "Computerized morphodensitometric analysis of blood cells from individuals exposed to low-dose radiation", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180785

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