Substrate-induced quenching-based measurement of glycerol

Ashutosh Sharma; Nigel S. M. Quantrill

doi:10.1117/12.180781

21 July 1994 Substrate-induced quenching-based measurement of glycerol

Ashutosh Sharma, Nigel S. M. Quantrill

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180781
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Glycerol concentrations are measured using a novel photo reaction between an excited fluorophore and NADH, the latter being generated due to the reduction of NAD⁺ in an enzymatic reaction between glycerol dehydrogenase (GlDH) and glycerol. The results are treated using a modified Stern-Volmer type relation and at equilibrium, a SIQ quenching constant of 9.78 (+/- 2.05) M^-1 is obtained for the substrate induced quenching of thionine by glycerol. The reported method was investigated over the glycerol concentration range of 0 - 100 mM and offers a detection limit of 0.4 mM.

Citation Download Citation

Ashutosh Sharma and Nigel S. M. Quantrill "Substrate-induced quenching-based measurement of glycerol", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180781

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