pH as a relevant factor for sensitizer state and cell photodynamic damage: spectroscopy and microphotometry

Elena B. Chernyaeva; Marina Yu. Poroshina; L. V. Zhorina; A. V. Agronskaya; Andrey Yuri Chikishev; V. A. Schinov; S. Yu. Ardgantsev; Maria G. Galpern

doi:10.1117/12.197451

2 January 1995 pH as a relevant factor for sensitizer state and cell photodynamic damage: spectroscopy and microphotometry

Elena B. Chernyaeva, Marina Yu. Poroshina, L. V. Zhorina, A. V. Agronskaya, Andrey Yuri Chikishev, V. A. Schinov, S. Yu. Ardgantsev, Maria G. Galpern

Author Affiliations +

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197451
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

Photodynamic therapy (PDT) of cancer is an intensively developing modality of cancer treatment based on the phototoxic action of some dyes retained selectively in tumor tissues. One of the final goals of the studies of fundamental mechanism of PDT is the rational choice of the photosensitizer (PS). To be able to do this one should understand the basic relationship between the structure of the sensitizing molecule and the efficiency of the selective photodynamic damage eventually induced in tumorous tissues by the combined action of the PS and light. Among the variety of physico-chemical factors influencing the photodynamic processes at the cellular level we have chosen the value of pH that is important both as a microenvironmental characteristic and as a parameter of cell physiology during the process of the photodynamically induced cell death. The data reported in this paper concern both roles of pH: pH as a microenvironmental factor influencing the state of the sensitizing molecule, and cytoplasmic pH as a factor relevant for the ability of the cell to resist photodynamic action. As the sensitizer one of the second generation PS was chosen -- tetrasulfonated aluminum phthalocyanine (AlPcS₄). The experimental technique used included stationary and time- resolved fluorescence spectroscopy and microfluorometry, numerical spectra deconvolution absorption spectroscopy and pH microphotometry of single cells.

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Elena B. Chernyaeva, Marina Yu. Poroshina, L. V. Zhorina, A. V. Agronskaya, Andrey Yuri Chikishev, V. A. Schinov, S. Yu. Ardgantsev, and Maria G. Galpern "pH as a relevant factor for sensitizer state and cell photodynamic damage: spectroscopy and microphotometry", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197451

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KEYWORDS

Fluorescence spectroscopy

Photodynamic therapy

Picosecond phenomena

Spectroscopy

Cancer

Molecules

Oncology

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