Detection of triglycerides using immobilized enzymes in food and biological samples

Ashish Raichur; Abiodun Lesi; Henrik Pedersen

doi:10.1117/12.238802

24 April 1996 Detection of triglycerides using immobilized enzymes in food and biological samples

Ashish Raichur, Abiodun Lesi, Henrik Pedersen

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238802
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A scheme for the determination of total triglyceride (fat) content in biomedical and food samples is being developed. The primary emphasis is to minimize the reagents used, simplify sample preparation and develop a robust system that would facilitate on-line monitoring. The new detection scheme developed thus far involves extracting triglycerides into an organic solvent (cyclohexane) and performing partial least squares (PLS) analysis on the NIR (1100 - 2500 nm) absorbance spectra of the solution. A training set using 132 spectra of known triglyceride mixtures was complied. Eight PLS calibrations were generated and were used to predict the total fat extracted from commercial samples such as mayonnaise, butter, corn oil and coconut oil. The results typically gave a correlation coefficient (r) of 0.99 or better. Predictions were typically within 90% and better at higher concentrations. Experiments were also performed using an immobilized lipase reactor to hydrolyze the fat extracted into the organic solvent. Performing PLS analysis on the difference spectra of the substrate and product could enhance specificity. This is being verified experimentally. Further work with biomedical samples is to be performed. This scheme may be developed into a feasible detection method for triglycerides in the biomedical and food industries.

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Ashish Raichur, Abiodun Lesi, and Henrik Pedersen "Detection of triglycerides using immobilized enzymes in food and biological samples", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238802

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KEYWORDS

Calibration

Absorbance

Absorption

Biomedical optics

Near infrared

Error analysis

Spectrophotometry

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