Characterization of microendoscopic imaging systems for tissue spectroscopy

Michael P. Houlne; Darren S. Hubbard; Darryl J. Bornhop

doi:10.1117/12.239535

10 May 1996 Characterization of microendoscopic imaging systems for tissue spectroscopy

Michael P. Houlne, Darren S. Hubbard, Darryl J. Bornhop

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239535
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Micro-endoscopes based on a fiber optic conduit and thin rod lens are investigated for applications in tissue diagnostics and studies of fluid transport in tissue-like matrices. The design, evaluation and implementation of these moderate to high resolution optical instruments is presented. The optical configuration employs a micro-endoscopic probe, an objective lens and a commercial color CCD. The flexible micro-endoscope is 2.65 meters in length, with an outer diameter of 1.5 mm and is employed to quantitatively measure EBT transport through a tissue-like substrate. The determined resolution is 20 line-pairs/mm (LP/mm). Calibration is accomplished by measuring absorbance of Eriochrome Black T dye against a reflective background. Fluorescence detection of fluorescein is evaluated using a thin rod lens micro- endoscope. The rod lens is 210 mm in length, 3 mm in outside diameter and can resolve 160 LP/mm. This resolution allows imaging of cellular organelles having a diameter of 2.8 mu;m. The limit of detection of the rod lens endoscopic system is approximately 20 pmol for fluorescein.

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Michael P. Houlne, Darren S. Hubbard, and Darryl J. Bornhop "Characterization of microendoscopic imaging systems for tissue spectroscopy", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239535

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