Improvement of three-dimensional resolution in confocal microscopy

Manuel Martinez-Corral; Pedro Andres; Enrique Silvestre-Mora; Juan Carlos Barreiro

doi:10.1117/12.231036

5 February 1996 Improvement of three-dimensional resolution in confocal microscopy

Manuel Martinez-Corral, Pedro Andres, Enrique Silvestre-Mora, Juan Carlos Barreiro

Proceedings Volume 2730, Second Iberoamerican Meeting on Optics; (1996) https://doi.org/10.1117/12.231036
Event: Second Iberoamerican Meeting on Optics, 1995, Guanajuato, Mexico

Abstract

A technique is presented for improving 3-D resolution in confocal scanning microscopy. The technique is based on the equal contribution to the image of the illuminating and the collecting lenses. It is proposed, then, to apodize such lenses with complementary filters. The combined action of both filters produces a narrowness of the point spread function of the system both in the image plane, and along the optical axis.

Citation Download Citation

Manuel Martinez-Corral, Pedro Andres, Enrique Silvestre-Mora, and Juan Carlos Barreiro "Improvement of three-dimensional resolution in confocal microscopy", Proc. SPIE 2730, Second Iberoamerican Meeting on Optics, (5 February 1996); https://doi.org/10.1117/12.231036

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