Fluorescent imaging and quantitation of solid support-bound nucleic acids

Valery L. Bogdanov; Yu-Hui Rogers; Michael Boyce-Jacino

doi:10.1117/12.274346

22 May 1997 Fluorescent imaging and quantitation of solid support-bound nucleic acids

Valery L. Bogdanov, Yu-Hui Rogers, Michael Boyce-Jacino

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274346
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Recent advances in molecular biology have enabled the deposition of nucleic acids on solid phases to form arrays of oligonucleotides. Such arrays are being applied in milli to nanoscale molecular and biochemical analysis such as genetic mutation detection, gene expression quantitation and DNA sequencing using so-called DNA chip arrays. A major obstacle to continue use of such arrays is detection and analysis of the arrayed nucleic acids, nucleic acids, nucleic acids' targets that bind via hybridization to the arrays and the products of array-based biochemical reactions. We report here on the design and utility of our experimental set-up for analysis of surface bound, arrayed oligonucleotides, and our experience in detection and quantitation of multiple fluorescent labels bound to the surface through attachment, hybridization, and arrayed primer extension.

Citation Download Citation

Valery L. Bogdanov, Yu-Hui Rogers, and Michael Boyce-Jacino "Fluorescent imaging and quantitation of solid support-bound nucleic acids", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274346

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available