Evaluation of interacellular tamoxifen-induced fluorescence in tamoxifen-resistant human breast adenocarcinoma cells

Nathalie Bachmann; Muriel Barberi-Heyob; Marie-Pierre Gramain; Corinne Bour; Sophie Marchal; Robert Michel Parache; Francois H. Guillemin; Jean-Louis Merlin

doi:10.1117/12.297980

29 December 1997 Evaluation of interacellular tamoxifen-induced fluorescence in tamoxifen-resistant human breast adenocarcinoma cells

Nathalie Bachmann, Muriel Barberi-Heyob, Marie-Pierre Gramain, Corinne Bour, Sophie Marchal, Robert Michel Parache, Francois H. Guillemin, Jean-Louis Merlin

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297980
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

A tamoxifen resistant cell line (MCF7^TAM) was established from tamoxifen sensitive MCF-7 human adenocarcinoma cells expressing estrogen receptors. The resistant cell line was found to express estrogen receptors to similar level as the parent cell line but the receptors were found to be altered, having lost their ability to bind estradiol or tamoxifen. The fluorescence of eosin-tamoxifen ionic association was used to investigate intracellular location of tamoxifen in both sensitive and resistant cell lines. Fluorescence emission spectra of eosin, tamoxifen and eosin-tamoxifen complex ((lambda) _exc equals 480 nm) were analyzed and showed that maximal fluorescence intensity of the complex ((lambda) _em equals 540 nm) was four times higher than that of eosin alone while tamoxifen alone did not emit any fluorescence in this spectral range. In MCF-7 cells, tamoxifen was found to be diffusively located in the cytoplasm and nuclear fluorescence intensity was significantly lower. No difference was observed in fluorescence intensity or location in tamoxifen resistant cells, although it has been previously correlated with clinical responsiveness. Improvement of this fluorescence microscopy methodology appears necessary to provide accurate results taking into account the complexity of tamoxifen resistance molecular pathways.

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Nathalie Bachmann, Muriel Barberi-Heyob, Marie-Pierre Gramain, Corinne Bour, Sophie Marchal, Robert Michel Parache, Francois H. Guillemin, and Jean-Louis Merlin "Evaluation of interacellular tamoxifen-induced fluorescence in tamoxifen-resistant human breast adenocarcinoma cells", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297980

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