Near-field optical microscopy of bacteria thin sections

Nikolai P. Konnov; Vil B. Baiburin; Anatolyi A. Shcherbakov; Alina N. Malakhaeva; Yuri P. Volkov

doi:10.1117/12.297978

29 December 1997 Near-field optical microscopy of bacteria thin sections

Nikolai P. Konnov, Vil B. Baiburin, Anatolyi A. Shcherbakov, Alina N. Malakhaeva, Yuri P. Volkov

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297978
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Whole bacteria as well as thin sections were investigated in our laboratory by means of near field scanning optical microscope (NSOM). The main problem in NSOM operation is a control of distance between microscopy tip and sample surface. The bacteria thin section is a more preferable sample for NSOM investigation because of its flat surface. For increasing of thin sections' image contrast were used different light microscopy stainers (Eosin, Hematoxylin, etc.). We obtained images of thin sections of plague (Y. Pestis EV) and cholera (V. Cholerae). Lateral resolution in the investigation is about 300 angstroms.

Citation Download Citation

Nikolai P. Konnov, Vil B. Baiburin, Anatolyi A. Shcherbakov, Alina N. Malakhaeva, and Yuri P. Volkov "Near-field optical microscopy of bacteria thin sections", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297978

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available