Corneal haze induced by excimer laser photoablation in rabbits is reduced by preserved human amniotic membrane graft

Ming X. Wang; Trevor Gray; Pinnita Prabhasawat; Xiong Ma; William Culbertson; Richard Forster; Khalil Hanna M.D.; Scheffer C. G. Tseng

doi:10.1117/12.309422

1 June 1998 Corneal haze induced by excimer laser photoablation in rabbits is reduced by preserved human amniotic membrane graft

Ming X. Wang, Trevor Gray, Pinnita Prabhasawat, Xiong Ma, William Culbertson, Richard Forster, Khalil Hanna M.D., Scheffer C. G. Tseng

Author Affiliations +

Proceedings Volume 3246, Ophthalmic Technologies VIII; (1998) https://doi.org/10.1117/12.309422
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We conducted a study to determine if preserved human amniotic membrane can reduce corneal haze induced by excimer laser photoablation. Excimer photoablation was performed bilaterally on 40 New Zealand white rabbits with a 6 mm ablation zone and 120 micrometer depth (PTK) using the VISX Star. One eye was randomly covered with a preserved human amniotic membrane and secured using four interrupted 10 - 0 nylon sutures; the other eye served as control. The amniotic membranes were removed at one week, and the corneal haze was graded with a slit-lamp biomicroscopy by three masked corneal specialists (WC, KH and RF) biweekly for the ensuing 12 weeks. Histology and in situ TUNEL staining (for fragmented DNA as an index for apoptosis) was performed at days 1, 3 and 7 and at 12 weeks. One week after excimer photoablation, the amniotic membrane-covered corneas showed more anterior stromal edema, which resolved at the second week. A consistent grading of organized reticular corneal haze was noted among the three masked observers. Such corneal haze peaked at the seventh week in both groups. The amniotic membrane-covered group showed statistically significant less corneal haze (0.50 plus or minus 0.15) than the control groups (1.25 plus or minus 0.35) (p less than 0.001). The amniotic membrane-covered corneas had less inflammatory response at days 1 and 3, showing nearly nil DNA fragmentation on keratocytes on the ablated anterior stromal and less stromal fibroblast activation. There is less altered epithelial cell morphology and less epithelial hyperplasia at 1 week in these amniotic membrane-treated eyes. We concluded from this study that amniotic membrane matrix is effective in reducing corneal haze induced by excimer photoablation in rabbits and may have clinical applications.

Citation Download Citation

Ming X. Wang, Trevor Gray, Pinnita Prabhasawat, Xiong Ma, William Culbertson, Richard Forster, Khalil Hanna M.D., and Scheffer C. G. Tseng "Corneal haze induced by excimer laser photoablation in rabbits is reduced by preserved human amniotic membrane graft", Proc. SPIE 3246, Ophthalmic Technologies VIII, (1 June 1998); https://doi.org/10.1117/12.309422

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Air contamination

Cornea

Wound healing

Eye

Cell death

Excimers

Excimer lasers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years