Changes in stiffness of yeast cells during cell cycle by passability through micromachined channel arrays

Hiroko E. Kikuchi; Yukio Magariyama; Yuji Kikuchi

doi:10.1117/12.304379

26 March 1998 Changes in stiffness of yeast cells during cell cycle by passability through micromachined channel arrays

Hiroko E. Kikuchi, Yukio Magariyama, Yuji Kikuchi

Proceedings Volume 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications; (1998) https://doi.org/10.1117/12.304379
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Possibility of cell sorting by cellular deformability was examined using yeast cells and previously described microchannel arrays. Cells harvested at every hour during incubation were washed and suspended in sorbitol solution at a concentration of O.D. 0.3. An aliquot of each suspension was caused to flow through the microchannel concentration of O.D. 0.3. An aliquot of each suspension was caused to flow through the microchannel arrays by applying 20 cmH₂O suction. Cells at two hours of incubation could not enter into the microchannels, while cells at 4-5 hours of incubation could enter into the microchannels despite their larger size due to budding than the preceding ones and some few cells were observed to pass through 8 micrometers width microchannels. The number of cells that could enter into the microchannels decreased at 7-8 hours and re-increased at 9- 10 hours, but the synchronism in this second cycle appeared to decrease. Protoplasts prepared by treatment with zymolyase from cells at 4-5 hours of incubation showed no appreciable resistance to the microchannel passage.

Citation Download Citation

Hiroko E. Kikuchi, Yukio Magariyama, and Yuji Kikuchi "Changes in stiffness of yeast cells during cell cycle by passability through micromachined channel arrays", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); https://doi.org/10.1117/12.304379

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