Applying fiber optical methods for toxicological testing in vitro

Holger K. Maerz; Rainer Buchholz; Frank Emmrich; Frank Fink; Clive L. Geddes; Lutz Pfeifer; Ferdinand Raabe; Thomas-Helmut Scheper; Elizabeth Ulrich; Uwe Marx

doi:10.1117/12.346746

21 April 1999 Applying fiber optical methods for toxicological testing in vitro

Holger K. Maerz, Rainer Buchholz, Frank Emmrich, Frank Fink, Clive L. Geddes, Lutz Pfeifer, Ferdinand Raabe, Thomas-Helmut Scheper, Elizabeth Ulrich, Uwe Marx

Author Affiliations +

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346746
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The new medical developments, e.g. immune therapy, patient oriented chemotherapy or even gene therapy, create a questionable doubt to the further requirement of animal test. Instead the call for humanitarian reproductive in vitro models becomes increasingly louder. Pharmaceutical usage of in vitro has a long proven history. In cancer research and therapy, the effect of chemostatica in vitro in the so-called oncobiogram is being tested; but the assays do not always correlate with in vivo-like drug resistance and sensitivity. We developed a drug test system in vitro, feasible for therapeutic drug monitoring by the combination of tissue cultivation in hollow fiber bioreactors and fiber optic sensors for monitoring the pharmaceutical effect. Using two fiber optic sensors - an optical oxygen sensor and a metabolism detecting Laserfluoroscope, we were able to successfully monitor the biological status of tissue culture and the drug or toxic effects of in vitro pharmaceutical testing. Furthermore, we developed and patented a system for monitoring the effect of minor toxic compounds which can induce Sick Building Syndrome.

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Holger K. Maerz, Rainer Buchholz, Frank Emmrich, Frank Fink, Clive L. Geddes, Lutz Pfeifer, Ferdinand Raabe, Thomas-Helmut Scheper, Elizabeth Ulrich, and Uwe Marx "Applying fiber optical methods for toxicological testing in vitro", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346746

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