Comparative microscopy study of Vibrio cholerae flagella

Nikolai P. Konnov; Vil B. Baiburin; Svetlana P. Zadnova; Uryi P. Volkov

doi:10.1117/12.350626

17 June 1999 Comparative microscopy study of Vibrio cholerae flagella

Nikolai P. Konnov, Vil B. Baiburin, Svetlana P. Zadnova, Uryi P. Volkov

Author Affiliations +

Proceedings Volume 3607, Scanning and Force Microscopies for Biomedical Applications; (1999) https://doi.org/10.1117/12.350626
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

A fine structure of bacteria flagella is an important problem of molecular cell biology. Bacteria flagella are the self-assembled structures that allow to use the flagellum protein in a number of biotechnological applications. However, at present, there is a little information about high resolution scanning probe microscopy study of flagellum structure, in particular, about investigation of Vibrio cholerae flagella. In our lab have been carried out the high resolution comparative investigation of V. cholerae flagella by means of various microscopes: tunneling (STM), scanning force (SFM) and electron transmission. As a scanning probe microscope is used designed in our lab versatile SPM with replaceable measuring heads. Bacteria were grown, fixed and treated according to the conventional techniques. For STM investigations samples were covered with Pt/Ir thin films by rotated vacuum evaporation, in SFM investigations were used uncovered samples. Electron microscopy of the negatively stained bacteria was used as a test procedure.

Citation Download Citation

Nikolai P. Konnov, Vil B. Baiburin, Svetlana P. Zadnova, and Uryi P. Volkov "Comparative microscopy study of Vibrio cholerae flagella", Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); https://doi.org/10.1117/12.350626

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