Marie Hamaoui,1 Jean-Marie A. Parel,1 Fabrice Manns,1 Hassan Tahi,2 Arthur Ho,3 Brien Holden3
1Bascom Palmer Eye Institute/Univ. of Miami School of Medicine and Univ. of Miami (United States) 2Bascom Palmer Eye Institute/Univ. of Miami School of Medicine and Univ. of Liege (United States) 3Univ. of New South Wales (Australia)
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The paper reviews two techniques being developed for ex-vivo testing of crystalline lens substitutes. The techniques may be used to test polymers developed for the Restore Accommodation (RA) project. Harvested rabbit and human lenses were used in preliminary tests to determine the feasibility of topographical analysis of the crystalline lens and ex-vivo cell culturing of the lens capsule. Lens capsules were cultured to test formation of posterior capsule opacification (PCO) in the presence of RA polymers. Feasibility of analyzing lens shape with the PARTM Sys Corneal Topography System was determined using whole lenses. Preliminary results indicate that topography and cell culturing of lens capsules may be used to evaluate RA polymers.
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Marie Hamaoui, Jean-Marie A. Parel, Fabrice Manns, Hassan Tahi, Arthur Ho, Brien Holden, "Ex-vivo testing of crystalline lens substitutes: a pilot study," Proc. SPIE 3908, Ophthalmic Technologies X, (7 June 2000); https://doi.org/10.1117/12.387515