Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner

Katsumasa Fujita; Tomoyuki Kaneko; Osamu Nakamura; Masahito Oyamada; Tetsuro Takamatsu; Satoshi Kawata

doi:10.1117/12.384225

27 April 2000 Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, Satoshi Kawata

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384225
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A real-time confocal multiphoton fluorescence microscope was developed to observe Ca²⁺ dynamics in living rat- cardiac muscle cells. The real-time imaging was achieved by multifocus excitation of a specimen with a rotating microlens-array disk. A pinhole-array disk for confocal detection was introduced in the microscope to improve the spatial resolution and the contrast of fluorescence images. Ca²⁺ wave and Ca²⁺ transient in cultured rat- cardiac cells were successfully observed with the developed microscope.

Citation Download Citation

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, and Satoshi Kawata "Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384225

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