Direct observation of supramolecular structures of biorelated materials by atomic force microscopy

Hiroyasu Yamaguchi; Kei Kubota; Akira Harada

doi:10.1117/12.383353

21 April 2000 Direct observation of supramolecular structures of biorelated materials by atomic force microscopy

Hiroyasu Yamaguchi, Kei Kubota, Akira Harada

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383353
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We have prepared DNA catenanes and studied the topological structures of DNA catenanes by atomic force microscopy (AFM) and electrophoresis. Nicked DNA was synthesized by the addition of DNASE I to a solution of plasmid pBR322. Catenated DNA molecules were prepared by the reaction of topoisomerase I with nicked DNA. Catenation reactions were monitored by the agarose gel electrophoresis. A droplet of solution, containing DNA catenanes extracted from the band of 1.0 percent agarose gel electrophoresis by a centrifugal filter device, was applied to a freshly cleaved mica surface. After drying the specimens, AFM measurements were carried out by using a silicon cantilever. The single molecule images of DNA catenanes were clearly observed for the first time by AFM using a tapping mode at room temperature and in an ambient atmosphere.

Citation Download Citation

Hiroyasu Yamaguchi, Kei Kubota, and Akira Harada "Direct observation of supramolecular structures of biorelated materials by atomic force microscopy", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383353

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available