Scanning acoustic microscopy reveals distinct motility domains in living cells

Ilonka Karl; Juergen Bereiter-Hahn

doi:10.1117/12.434181

24 July 2001 Scanning acoustic microscopy reveals distinct motility domains in living cells

Ilonka Karl, Juergen Bereiter-Hahn

Proceedings Volume 4335, Advanced Nondestructive Evaluation for Structural and Biological Health Monitoring; (2001) https://doi.org/10.1117/12.434181
Event: 6th Annual International Symposium on NDE for Health Monitoring and Diagnostics, 2001, Newport Beach, CA, United States

Abstract

In the middle of the 90s, the image processing and analysing method subtraction scanning acoustic microscopy (SubSAM) was developed to display and quantify cell surface motility. Since then, the approach of this method supported already existing models and provided new insights into the regulation of cell surface motility. The investigations with SubSAM revealed that cell surface motility is modulated by tension and is regulated by known F-actin reorganising signal transduction pathways via the small GTP-binding protein Rac and protein kinase C. Beyond that, the in vitro cell surface motility as revealed by SubSAM corelates with cell transformation respectively the invasive or metastatic potential. The enhancement of cell surface motility is always accompanied by characteristic actin reorganisation: loss of stress fibers and the formation of cortical F-actin knots or larger aggregates. From these observations a model has been developed for the regulation of cell surface motility and an addtitional mechanism for cell surface deformation based on myosin-dependent F-actin aggregation is proposed.

Citation Download Citation

Ilonka Karl and Juergen Bereiter-Hahn "Scanning acoustic microscopy reveals distinct motility domains in living cells", Proc. SPIE 4335, Advanced Nondestructive Evaluation for Structural and Biological Health Monitoring, (24 July 2001); https://doi.org/10.1117/12.434181

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