A novel method of synchronization of separation and analysis is described, which use the mode-filtered light detection as an on line detector of capillary electrophoresis, furthermore, corresponding to this method an instrument has been established. Capillary electrophoresis runs in an annular column, which is constructed by a naked optical fiber inserted into a fused silica capillary, instead of a round capillary, instead of a round capillary. Moreover, the annular column is not only a vessel for electrophoresis but also a sensor to the mode-filtered light. Along the annular column side several detection channels are set up to gather the mode-filtered light and transmit it to CCD, so every channel provides the information of the sample form where it is located. Using the capillary isotachophoresis in this annular column, a sample containing alanine and glycin is separated and analyzed simultaneously by the multi-channel mode-filtered light detection.
|