Synchronization of separation and analysis based on multichannel mode-filtered light detection with CE

Zheng Zhou; Kemin Wang; Xiaohai Yang; Dilan Qin; Du Li

doi:10.1117/12.440187

14 September 2001 Synchronization of separation and analysis based on multichannel mode-filtered light detection with CE

Zheng Zhou, Kemin Wang, Xiaohai Yang, Dilan Qin, Du Li

Author Affiliations +

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440187
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

A novel method of synchronization of separation and analysis is described, which use the mode-filtered light detection as an on line detector of capillary electrophoresis, furthermore, corresponding to this method an instrument has been established. Capillary electrophoresis runs in an annular column, which is constructed by a naked optical fiber inserted into a fused silica capillary, instead of a round capillary, instead of a round capillary. Moreover, the annular column is not only a vessel for electrophoresis but also a sensor to the mode-filtered light. Along the annular column side several detection channels are set up to gather the mode-filtered light and transmit it to CCD, so every channel provides the information of the sample form where it is located. Using the capillary isotachophoresis in this annular column, a sample containing alanine and glycin is separated and analyzed simultaneously by the multi-channel mode-filtered light detection.

Citation Download Citation

Zheng Zhou, Kemin Wang, Xiaohai Yang, Dilan Qin, and Du Li "Synchronization of separation and analysis based on multichannel mode-filtered light detection with CE", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440187

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