Knowledge-based image processing for on-off type DNA microarray

Jong Dae Kim; Seo Kyu Kim; Jeong Sik Cho; Jongwon Kim

doi:10.1117/12.469458

5 June 2002 Knowledge-based image processing for on-off type DNA microarray

Jong Dae Kim, Seo Kyu Kim, Jeong Sik Cho, Jongwon Kim

Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469458
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

This paper addresses the image processing technique for discriminating whether the probes are hybrized with target DNA in the Human Papilloma Virus (HPV) DNA Chip designed for genotyping HPV. In addition to the probes, the HPV DNA chip has markers that always react with the sample DNA. The positions of probe-dots in the final scanned image are fixed relative to the marker-dot locations with a small variation according to the accuracy of the dotter and the scanner. The probes are duplicated 4 times for the diagnostic stability. The prior knowledges such as the maker relative distance and the duplication information of probes is integrated into the template matching technique with the normalized correlation measure. Results show that the employment of both of the prior knowledges is to simply average the template matching measures over the positions of the markers and probes. The eventual proposed scheme yields stable marker locating and probe classification.

Citation Download Citation

Jong Dae Kim, Seo Kyu Kim, Jeong Sik Cho, and Jongwon Kim "Knowledge-based image processing for on-off type DNA microarray", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469458

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available