Parallel immunoassays on HydroGel biochips using microspot arrays

Martin Sommer; Cheng Wang; Henry Lisoukov; Robert M. Cavallo; Thomas E. Miller; Jocelyn Burke

doi:10.1117/12.472059

21 June 2002 Parallel immunoassays on HydroGel biochips using microspot arrays

Martin Sommer, Cheng Wang, Henry Lisoukov, Robert M. Cavallo, Thomas E. Miller, Jocelyn Burke

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472059
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Parallel immunoassay enable simultaneous detection of many analytes forma small volume of sample fluid. The use of exceedingly small quantities of capture antibody immobilized in microspots facilitates ambient analyte assay conditions. Under these assy conditions, the fractional occupancy of capture antibody is independent of sample volume. Analyte concentrations are thus measured with greater sensitivity and potentially greater speed. The small drop size delivered by the Packard BioChip Arrayer, a non-contact piezoelectric robotic dispenser, enables the production of arrays consisting of high reproducible microspots of antibodies. The Packard HyrdoGel coated slide is a porous substrate based on a polymer matrix that provides a 3D hydrophilic environment similar to free solution suitable for biomolecular interactions. This substrate has been used to develop fluorescence-based detection immunoassay in multiplex with high sensitivity and offers advantages of increased probe capacity, functionality and accessability for other biomolecular screening applications. Images are captured with the ScanArray confocal laserscanner, which offers the use to choose from a wide range of fluorescent dyes with high resolution and sensitivity, and analyzed with QuantArray microarray analysis software, which enables researchers to easily and accurately visualize and quantitate gene expression and proteomics data.

Citation Download Citation

Martin Sommer, Cheng Wang, Henry Lisoukov, Robert M. Cavallo, Thomas E. Miller, and Jocelyn Burke "Parallel immunoassays on HydroGel biochips using microspot arrays", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472059

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