A highly sensitive chemiluminescence method for early diagnosis of radiation damage

Yadong Wei; Da Xing; Debin Zhu; Yonghong He; Hongbo Fu

doi:10.1117/12.572441

29 July 2004 A highly sensitive chemiluminescence method for early diagnosis of radiation damage

Yadong Wei, Da Xing, Debin Zhu, Yonghong He, Hongbo Fu

Author Affiliations +

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572441
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

Chemiluminescence (CL) is a highly sensitive detection method with broad biological applications. In this work, a CL detection system is developed and applied in detection the CL from human lymphocyte induced with Co-60 irradiation, for the first time. The radiation damage of lymphocyte was detected by cell counting and MTT method for various irradiation doses. The results show a good correlation between the CL intensity and the radiation-induced damage of lymphocyte. Cell activity increased gradually with the increase of radiate dose, when the dose was under 3Gy. When the radiate dose was above 3Gy, the results were contrary. The cell counting results corresponded well with the MTT method. The CL detection method, thus, may provide an alternative way in evaluation of radiation damage.

Citation Download Citation

Yadong Wei, Da Xing, Debin Zhu, Yonghong He, and Hongbo Fu "A highly sensitive chemiluminescence method for early diagnosis of radiation damage", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572441

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