A novel UV-laser source for fluorescence excitation of proteins

Mikael Tiihonen; Valdas Pasiskevicius; Fredrik Laurell; Mikael Lindgren

doi:10.1117/12.568436

29 December 2004 A novel UV-laser source for fluorescence excitation of proteins

Mikael Tiihonen, Valdas Pasiskevicius, Fredrik Laurell, Mikael Lindgren

Proceedings Volume 5617, Optically Based Biological and Chemical Sensing for Defence; (2004) https://doi.org/10.1117/12.568436
Event: European Symposium on Optics and Photonics for Defence and Security, 2004, London, United Kingdom

Abstract

A novel, compact and robust UV laser has been developed for laser induced fluorescence spectroscopy of biomolecules in the spectral region from 290 nm to 345 nm. It was based on a frequency-doubled passively Q-switched Nd:YAG laser, emitting at 532 nm, which was pumping a periodically poled KTiOPO₄ optical parametric oscillator with intra-cavity sum-frequency mixing in a BBO crystal. The output was generated in two branches in the UV, 293 nm and 343 nm, with pulse widths of 1.8 ns and pulse repetition rate of 100 Hz. These wavelengths were then used for fluorescence experiments of bioagents.

Citation Download Citation

Mikael Tiihonen, Valdas Pasiskevicius, Fredrik Laurell, and Mikael Lindgren "A novel UV-laser source for fluorescence excitation of proteins", Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); https://doi.org/10.1117/12.568436

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