Refractive index determination from multiphoton and confocal spherical aberration microscopy

Peter T. Fwu; Wen Lo; Chen-Yuan Dong

doi:10.1117/12.587255

30 March 2005 Refractive index determination from multiphoton and confocal spherical aberration microscopy

Peter T. Fwu, Wen Lo, Chen-Yuan Dong

Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.587255
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Refractive index mismatch induced spherical aberration (RIMISA) is a ubiquitous problem for in-depth optical biological imaging and it is an effect most researchers like to avoid. Associated with RIMISA is a broadening of the point-spread-function (PSF) which leads to a degradation of image resolution and the loss of specimen signal. It is these features that render RIMISA to be useful for one important application in biological application, the determination of sample refractive index. We will show how RIMISA under multiphoton and confocal microscopy can be used to determine the refractive indices of uniformly luminescent specimens and a comparison will be made between the multiphoton and confocal approaches.

Citation Download Citation

Peter T. Fwu, Wen Lo, and Chen-Yuan Dong "Refractive index determination from multiphoton and confocal spherical aberration microscopy", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.587255

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