The Use Of Fluorescence Quenching To Measure Oxygen Concentration

M. E. Cox; B. Dunn

doi:10.1117/12.950729

14 January 1986 The Use Of Fluorescence Quenching To Measure Oxygen Concentration

M. E. Cox, B. Dunn

Proceedings Volume 0576, Optical Fibers in Medicine and Biology I; (1986) https://doi.org/10.1117/12.950729
Event: 29th Annual Technical Symposium, 1985, San Diego, United States

Abstract

The method of fluorescence quenching is used to measure the concentration of molecular oxygen. The method is rapid, reversible, and does not consume oxygen. The method may provide the basis for a unique biomedical sensor. The key to developing such a device lies in the choice of a fluorophor/polymer composite matrix having the desired optical and transport properties. Experimental results will be presented for certain parameters essential for assessing device development. The properties of interest include the kinetics of fluorescence quenching, the biomolecular rate constants, the temperature dependence of oxygen solubility and diffusivity in the composite matrix, and the oxygen diffusion coefficient. Poly(dimethyl siloxane) [PDMS] was chosen as the polymer host because it is biocompatible, hydrophobic, has a high diffusivity for the simple gases, and is easily bonded to fused silica. 9,10-diphenyl anthracene [9,10-D] was selected since it is readily soluble in a number of organic solvents, has an excitation spectrum in the near UV, an emission spectrum in the visible, a long fluorescence lifetime, and a high quantum yield. When incorporated into PDMS, the optical spectra of 9,10-D does not alter appreciably. The response time of the device is determined by the solution/diffusion kinetics of oxygen in PDMS. The solubility of oxygen in PDMS decreases with increasing temperature and an enthalpy of solution of off = -3.0 kcal/mole. (1) The diffusion of oxyzen in PDMS is found to obey an Arrhenius relation over the temperature range of 5'C to 450C with D = Do exp (-ED/RT) (2) where Do = 0.115 cm²/s (3) and ED = 4.77 kcal/mole. (4) Results of these studies indicate that an appropriate device, based on a fluorophor/polymer composite, for the measurement of oxygen concentration should be sensitive over those ranges which are important for physiological monitoring.

Citation Download Citation

M. E. Cox and B. Dunn "The Use Of Fluorescence Quenching To Measure Oxygen Concentration", Proc. SPIE 0576, Optical Fibers in Medicine and Biology I, (14 January 1986); https://doi.org/10.1117/12.950729

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