Biosensing and -imaging with enantiomeric luminescent conjugated polythiophenes using multiphoton excitation

Mikael Lindgren; Frantz Stabo-Eeg; Edrun A. Schnell; K. Peter R. Nilsson; Per Hammarstrom; Olle Inganaes

doi:10.1117/12.624438

18 August 2005 Biosensing and -imaging with enantiomeric luminescent conjugated polythiophenes using multiphoton excitation

Mikael Lindgren, Frantz Stabo-Eeg, Edrun A. Schnell, K. Peter R. Nilsson, Per Hammarstrom, Olle Inganaes

Author Affiliations +

Proceedings Volume 5935, Linear and Nonlinear Optics of Organic Materials V; 59350E (2005) https://doi.org/10.1117/12.624438
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

We report on the initial time-resolved luminescense and nonlinear absorption properties of two polythiophenes 3-substituted with chiral charged amino acid-derivatized substituents, POWT and POMT. The photo-physical characterization yielded quantum efficiency typically in the range 0.01 - 0.1, however, with two-photon absorption cross-section better than or similar to a typical two-photon reference chromophore, such as fluorescein. They were tested as conformational sensitive optical probes for the recording of pH-induced conformational changes of synthetic peptides, proteins and samples of protein amyloid fibrils characteristic of amyloid related diseases. Particularly, the POMT polyelectrolyte with the L-enantiomeric side chains is shown to favor this induction of well defined structure as judged by the circular dichroic signal as well as a stronger enhancement of luminescense for the L-form over the D-form complex. Furthermore, time-resolved fluorescense and two-photon induced fluorescence both also showed a difference in the complexation with the D and L form. This shows that the multi-photon excitation path can be an efficient means for chiral recognition of biomolecular complexes. It is demonstrated how the conjugated polyelectrolyte L-POMT can be used to spectrally image the formation of amyloid fibrils of insulin using both one- and two-photon absorption based fluorescence imaging.

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Mikael Lindgren, Frantz Stabo-Eeg, Edrun A. Schnell, K. Peter R. Nilsson, Per Hammarstrom, and Olle Inganaes "Biosensing and -imaging with enantiomeric luminescent conjugated polythiophenes using multiphoton excitation", Proc. SPIE 5935, Linear and Nonlinear Optics of Organic Materials V, 59350E (18 August 2005); https://doi.org/10.1117/12.624438

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