High fluence laser irradiation induces reactive oxygen species generation in human lung adenocarcinoma cells

Fang Wang; Da Xing; Tong-Sheng Chen

doi:10.1117/12.710934

27 October 2006 High fluence laser irradiation induces reactive oxygen species generation in human lung adenocarcinoma cells

Fang Wang, Da Xing, Tong-Sheng Chen

Author Affiliations +

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473C (2006) https://doi.org/10.1117/12.710934
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Low-power laser irradiation (LPLI) has been used for therapies such as curing spinal cord injury, healing wound et al. Yet, the mechanism of LPLI remains unclear. Our previous study showed that low fluences laser irradiation induces human lung adenocarcinoma cells (ASTC-a-1) proliferation, but high fluences induced apoptosis and caspase-3 activation. In order to study the mechanism of apoptosis induced by high fluences LPLI further, we have measured the dynamics of generation of reactive oxygen species (ROS) using H₂DCFDA fluorescence probes during this process. ASTC-a-1 cells apoptosis was induced by He-Ne laser irradiation at high fluence of 120J/cm². A confocal laser scanning microscope was used to perform fluorescence imaging. The results demonstrated that high fluence LPLI induced the increase of mitochondria ROS. Our studies contribute to clarify the biological mechanism of high fluence LPLI-induced cell apoptosis.

Citation Download Citation

Fang Wang, Da Xing, and Tong-Sheng Chen "High fluence laser irradiation induces reactive oxygen species generation in human lung adenocarcinoma cells", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473C (27 October 2006); https://doi.org/10.1117/12.710934

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