Decompose touching blood cells based on the information of weak edges and object shape

Weixing Wang

doi:10.1117/12.710726

27 October 2006 Decompose touching blood cells based on the information of weak edges and object shape

Weixing Wang

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473J (2006) https://doi.org/10.1117/12.710726
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

This paper presents how to split touching blood cells. The algorithm mainly consists of three sub-algorithms; they are Canny edge based segmentation, the algorithm of splitting based on valley edge (weak edges) detection and the algorithm for splitting based on shape information. It first traces blood cell boundaries based on discontinuities, then splits touching parts of blood cells based on slow variations of gray information in touching parts, and finally splits the remaining touching parts, where no gray information can be used, by using morphological mathematics method (e.g. distance map) combining blood cell shape information (information is classified by using fuzzy mathematics). The algorithm can automatically delineate blood cells not only on a simple image (a few blood cells touching) and also on a complex image (many cells touching together). The algorithm has been tested in a laboratory, it works well.

Citation Download Citation

Weixing Wang "Decompose touching blood cells based on the information of weak edges and object shape", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473J (27 October 2006); https://doi.org/10.1117/12.710726

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available