Frame self-division applied in analysis of intrinsic signal optical imaging data

Weihua Luo; Pengcheng Li; Li Zhang; Xiaohua Lv; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.645570

3 March 2006 Frame self-division applied in analysis of intrinsic signal optical imaging data

Weihua Luo, Pengcheng Li, Li Zhang, Xiaohua Lv, Shaoqun Zeng, Qingming Luo

Proceedings Volume 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III; 60850E (2006) https://doi.org/10.1117/12.645570
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In intrinsic signal optical imaging data, the response signal related cortical activity is very small compared to the background signal intensity and accompanied with unwanted noise from several different sources. Although there are some sophisticated data analysis techniques developed to achieve this critical demand, experience proved that no method perfect enough to be optimal for all experimental situations. Here we introduced a simple and practicable pre-processing method to attenuate the global noise source, such as the fluctuation of the illuminating light, changes of the animal's blood pressure and vasomotor oscillations. Before image processing to characterize the spatiotemporal response from cortical activity, each frame of image data was divided by the average or median of the current frame data. We applied the method to both simulated data and experimental optical imaging data to evaluate the validity and limitation.

Citation Download Citation

Weihua Luo, Pengcheng Li, Li Zhang, Xiaohua Lv, Shaoqun Zeng, and Qingming Luo "Frame self-division applied in analysis of intrinsic signal optical imaging data", Proc. SPIE 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III, 60850E (3 March 2006); https://doi.org/10.1117/12.645570

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available