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This study evaluated ex vivo the influence of the number of gel/LED-laser applications/activations on cervical
microleakage of two different barrier materials used for protection during whitening of endodontically treated teeth.
Eighty-four canines were instrumented and obturated with epoxy resin sealer. The seal was removed 2 mm beyond the
cemento-enamel junction for barrier placement and the teeth were divided into two groups of 40 teeth each: G1, zinc
phosphate cement; G2, glass ionomer cement. The two groups were subdivided into 4 subgroups (n=10 each): I) no gel or
LED-laser application; II) one gel application and two LED-laser activations; III) two gel applications and four LED-laser
activations; IV) three gel applications and six LED-laser activations. The teeth were immersed in India ink for 7
days, decalcified and cleared. Cervical microleakage was quantified with a measurement microscope. Statistical analysis
showed that zinc phosphate caused significantly lower microleakage than glass ionomer cement (presented microleakage
in all subgroups). However, after two (p<0.01) and three (p<0.001) applications of gel, there was statistially significant
microleakage in zinc phosphate barriers. Based on the present results, it can be concluded that cervical barriers with zinc
phosphate cement show less cervical microleakage and that two or more applications/activations of gel/LED-laser
significantly increase microleakage.
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