Methods and instruments for continuous-flow PCR on a chip

Claudia Gärtner; Richard Klemm; Holger Becker

doi:10.1117/12.713797

22 January 2007 Methods and instruments for continuous-flow PCR on a chip

Claudia Gärtner, Richard Klemm, Holger Becker

Proceedings Volume 6465, Microfluidics, BioMEMS, and Medical Microsystems V; 646502 (2007) https://doi.org/10.1117/12.713797
Event: MOEMS-MEMS 2007 Micro and Nanofabrication, 2007, San Jose, California, United States

Abstract

PCR is the most commonly used method to identify DNA segments. Several methods have been used to miniaturize PCR and perform it in a microfluidic chip. A unique approach is the continuous-flow PCR, where the conventional thermocycling is replaced by pumping the sample through a channel which meanders over stationary temperature zones, allowing fast temperature changes in the sample due to the low thermal mass as well as a continuous production of PCR products. In this paper we present a system comprising a polymer microfluidic chip, a thermocycler unit and the protocols necessary to perform fast continuous-flow PCR including experimental results in comparison with a conventional PCR system.

Citation Download Citation

Claudia Gärtner, Richard Klemm, and Holger Becker "Methods and instruments for continuous-flow PCR on a chip", Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 646502 (22 January 2007); https://doi.org/10.1117/12.713797

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