Measurement of human embryonic stem cell in the growing cycle

X. Li; L. Zhao; Steve K. W. Oh; W. K. Chong; J. K. Ong; Allen K. Chen; Andre B. H. Choo

doi:10.1117/12.814615

3 October 2008 Measurement of human embryonic stem cell in the growing cycle

X. Li, L. Zhao, Steve K. W. Oh, W. K. Chong, J. K. Ong, Allen K. Chen, Andre B. H. Choo

Author Affiliations +

Proceedings Volume 7155, Ninth International Symposium on Laser Metrology; 715535 (2008) https://doi.org/10.1117/12.814615
Event: Ninth International Symposium on Laser Metrology, 2008, Singapore, Singapore

Abstract

A measurement and imaging system has been developed for in-line continuous measurement of live, unmodified, human embryonic stem cells (hESC). The measurement will not affect cell growth, structure, sterility and suitability for clinical use. The stem cell imaging system (SCIS) can be used to support the optimization of automated stem cell growth for invitro study and for high-volume bio-manufacture. This paper present the experimental and analysis for the optimization of system parameters. A non-linear lighting is developed to obtain a clear images. The individual cluster can be traced from day one to day two. The whole system is calibrated with measurement microscope and haemacytometer. The measurement accuracy is better than 90%.

Citation Download Citation

X. Li, L. Zhao, Steve K. W. Oh, W. K. Chong, J. K. Ong, Allen K. Chen, and Andre B. H. Choo "Measurement of human embryonic stem cell in the growing cycle", Proc. SPIE 7155, Ninth International Symposium on Laser Metrology, 715535 (3 October 2008); https://doi.org/10.1117/12.814615

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