Non-invasive measurements of hemoglobin + myoglobin, their oxygenation and NIR light pathlength in heart in vivo by diffuse reflectance spectroscopy

Eugene Gussakovsky; Olga Jilkina; Yanmin Yang; Valery Kupriyanov

doi:10.1117/12.807715

23 February 2009 Non-invasive measurements of hemoglobin + myoglobin, their oxygenation and NIR light pathlength in heart in vivo by diffuse reflectance spectroscopy

Eugene Gussakovsky, Olga Jilkina, Yanmin Yang, Valery Kupriyanov

Author Affiliations +

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 71612L (2009) https://doi.org/10.1117/12.807715
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The existing non-invasive optical methods of the hemoglobin (Hb) and myoglobin (Mb) estimation in cardiac tissues imply knowledge of the light pathlength (L) when various modifications of Lambert-Beer law for either spectrophotometry or light diffuse reflectance is applied. For Hb and/or Mb quantification in tissue, a few invasive (biochemical) approaches were applied. For L (differential pathlength factor; DPF) determination in tissue, special optical methods were used. No approaches have been proposed to simultaneously and non-invasively determine Hb/Mb and L in cardiac or other muscle tissues. In the present study, the first derivative of the NIR diffuse reflectance spectrum is shown to be effective in simultaneous determination of Hb+Mb concentration (in mM) and L (in mm) in cardiac tissue in vivo. The results showed that measured in a few minutes in a normal pig heart in vivo the total Hb+Mb concentration was 0.9-1.2 mM of heme, tissue oxygen saturation parameter (OSP) was approximately 65%, and DPF at 700-965 nm was of 2.7-2.8. At the experimental ischemia, total [Hb+Mb] decreased by 25%, OSP reduced to zero, while DPF did not change. These results correlated with the previously published. The method may be applied during open-heart surgery, heart studies ex vivo or to any muscle tissue to continuously and non-invasively monitor the [Hb+Mb] content and oxygenation as well as L, which may reflect the changes in tissue structure.

Citation Download Citation

Eugene Gussakovsky, Olga Jilkina, Yanmin Yang, and Valery Kupriyanov "Non-invasive measurements of hemoglobin + myoglobin, their oxygenation and NIR light pathlength in heart in vivo by diffuse reflectance spectroscopy", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71612L (23 February 2009); https://doi.org/10.1117/12.807715

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available