Determination of threshold average temperature for cell death in an in vitro retinal model using thermography

Michael L. Denton; Michael S. Foltz; Gary D. Noojin; Larry E. Estlack; Robert J. Thomas; Benjamin A. Rockwell

doi:10.1117/12.807861

12 February 2009 Determination of threshold average temperature for cell death in an in vitro retinal model using thermography

Michael L. Denton, Michael S. Foltz, Gary D. Noojin, Larry E. Estlack, Robert J. Thomas, Benjamin A. Rockwell

Author Affiliations +

Proceedings Volume 7175, Optical Interactions with Tissue and Cells XX; 71750G (2009) https://doi.org/10.1117/12.807861
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Even though laser exposures of 1 s or less are non-isothermal events, researchers have had to rely upon the isothermal treatise of Arrhenius to describe the laser damage rate processes. To fully understand and model thermal damage from short exposure to laser irradiation we need to experimentally obtain the temperature history of exposed cells and correlate it with the cellular damage outcomes. We have recorded the thermal response of cultured retinal pigment epithelial cells in real-time with laser exposure using infrared imaging (thermography). These images were then overlaid with fluorescence images indicating cell death taken 1 hr post laser exposure. The image overlays allowed us to define the thermal history of cells at the boundary (threshold) of laser-induced death. We have found a correlation between the onset of cell death and the average temperature over the course of the laser exposure.

Citation Download Citation

Michael L. Denton, Michael S. Foltz, Gary D. Noojin, Larry E. Estlack, Robert J. Thomas, and Benjamin A. Rockwell "Determination of threshold average temperature for cell death in an in vitro retinal model using thermography", Proc. SPIE 7175, Optical Interactions with Tissue and Cells XX, 71750G (12 February 2009); https://doi.org/10.1117/12.807861

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