Lipids distribution imaging of lipid vesicles by multi-focus excitation CARS microscope

Takeo Minamikawa; Tsutomu Araki; Mamoru Hashimoto

doi:10.1117/12.808679

13 February 2009 Lipids distribution imaging of lipid vesicles by multi-focus excitation CARS microscope

Takeo Minamikawa, Tsutomu Araki, Mamoru Hashimoto

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718328 (2009) https://doi.org/10.1117/12.808679
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We demonstrated high-speed imaging of the distribution of DPPC (dipalmitoylphosphatidylcholine), d62-DPPC (deuterated DPPC), and DOPC (dioleoylphosphatidylcholine) lipids in a lipid vesicle with a multi-focus excitation CARS (coherent anti-Stokes Raman scattering) microscope using a microlens array scanner. By the multi-focus excitation, the dwell time is increased in proportion to the number of focal spots compared with a single beam scanning, and high-speed and high-quality CARS imaging is possible without increasing the peak power of each spot. We demonstrated the selectively visualization of DPPC and d62-DPPC lipid vesicles, in which the vesicles contain a type of lipid, by observing at 2840 cm^-1 and 2090 cm^-1. We also visualized the DOPC and DPPC lipids distribution in a lipid mixture vesicle observed at 1440 cm^-1 and 1655 cm^-1. The image acquisition time of 10 s/image at each Raman shift was realized. The signal ratio of 1440 cm^-1 and 1655 cm^-1 was locally intense on the lipid vesicle. It must be because the gel phase domain of DPPC lipids was exists in the DOPC lipids which were liquid-crystalline phase at room temperature.

Citation Download Citation

Takeo Minamikawa, Tsutomu Araki, and Mamoru Hashimoto "Lipids distribution imaging of lipid vesicles by multi-focus excitation CARS microscope", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718328 (13 February 2009); https://doi.org/10.1117/12.808679

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KEYWORDS

Raman spectroscopy

Digital signal processing

Microscopes

CARS tomography

Microlens array

Mode locking

Pulsed laser operation

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