Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification

J. Harris; G. McConnell

doi:10.1117/12.811160

27 February 2009 Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification

J. Harris, G. McConnell

Author Affiliations +

Proceedings Volume 7193, Solid State Lasers XVIII: Technology and Devices; 719327 (2009) https://doi.org/10.1117/12.811160
Event: SPIE LASE: Lasers and Applications in Science and Engineering, 2009, San Jose, California, United States

Abstract

We report a system for optical trapping, manipulation and imaging of live cells in close to real-time with a wide field of view. A fs-pulsed Ti:Sapphire source was used to trap cells whilst an Ar⁺ laser was used for confocal imaging. A single 10x/0.4NA lens and a dual scanning system enabled independent trapping, imaging and movement of cells over a field of view >1mm². We will present movies of controlled T cell and dendritic cell movement and interaction. This enables the functional study of cell-signalling processes that are fundamental to understanding the behaviour of the immune system and beyond.

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J. Harris and G. McConnell "Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification", Proc. SPIE 7193, Solid State Lasers XVIII: Technology and Devices, 719327 (27 February 2009); https://doi.org/10.1117/12.811160

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KEYWORDS

Imaging systems

Optical tweezers

Confocal microscopy

Luminescence

Absorption

Optical manipulation

Sapphire lasers

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