Optimum schemes for wavefront sensorless adaptive optics in microscopy

Delphine Débarre; Biru Wang; Tony Wilson; Martin J. Booth

doi:10.1117/12.810512

23 February 2009 Optimum schemes for wavefront sensorless adaptive optics in microscopy

Delphine Débarre, Biru Wang, Tony Wilson, Martin J. Booth

Proceedings Volume 7209, MEMS Adaptive Optics III; 720904 (2009) https://doi.org/10.1117/12.810512
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

In adaptive microscope systems, it is often desirable to dispense with the wavefront sensor and perform aberration correction through optimisation an appropriate quality metric, such as image brightness or sharpness. A sequence of trial aberrations is applied to the adaptive element and the metric values are calculated. The optimum aberration correction is derived from these measurements. An important choice in the design of these correction schemes is the modal aberration expansion. This choice may depend upon several factors, such as the deformable mirror, the optimisation metric, the aberration statistics or the image properties. We discuss these factors with particular reference to microscope imaging.

Citation Download Citation

Delphine Débarre, Biru Wang, Tony Wilson, and Martin J. Booth "Optimum schemes for wavefront sensorless adaptive optics in microscopy", Proc. SPIE 7209, MEMS Adaptive Optics III, 720904 (23 February 2009); https://doi.org/10.1117/12.810512

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