In vitro study of the effect of a pulsed 10.6 µm CO2 laser and fluoride on the reduction of carious lesions progression in bovine root dentin

Thaís M. Parisotto; Patrícia A. Sacramento; Marcelo C. Alves; Regina M. Puppin-Rontani; Maria Beatriz D. Gavião; Marinês Nobre-dos-Santos

doi:10.1117/12.842850

5 March 2010 In vitro study of the effect of a pulsed 10.6μm CO₂ laser and fluoride on the reduction of carious lesions progression in bovine root dentin

Thaís M. Parisotto, Patrícia A. Sacramento, Marcelo C. Alves, Regina M. Puppin-Rontani, Maria Beatriz D. Gavião, Marinês Nobre-dos-Santos

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Proceedings Volume 7549, Lasers in Dentistry XVI; 75490J (2010) https://doi.org/10.1117/12.842850
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The aim of the present study was to evaluate the combined in vitro effects a pulsed 10.6 μm CO₂ laser and fluoride on the reduction of carious lesion progression in root dentin. Sixty five slabs of previously demineralized bovine root dentin were assigned into five groups (n=13): control (no treatment), acidulated phosphate fluoride gel 1.23% (FFA), CO₂ Laser (L), FFA+L, L+FFA. The lasered groups were irradiated with 4.0J/cm2. After a 7 day pH cycling regime, the knoop hardness number (KHN) was determined by cross-sectional microhardness testing (5g, 5s, 10-60 μm, 10 μm interval). The data was analyzed by ANOVA and Student's t-test (α= 0.05). A significant interaction between KHN and the indentation depths was found (p<0.05). At 10-20 μm, FFA+L (KHN:12.12±0.95/13.07±1.03) and FFA (KHN:12.86±4.54/12.60±3.93) inhibited caries progression when compared to control group (KHN:8.76±0.95/9.50±1.03) (p<0.05), but did not differ with neither each other nor from group L (p>0.05). At 30 μm, the KHN was significantly higher than the control only in the FFA group (KHN:15.35±1.16). At 40 μm, the groups FFA (KHN: 15.87±3.76), L (KHN: 15.57±5.71) and L+FFA (KHN:15.50±5.08) were capable of significantly inhibiting caries progression, however they did not differ each other (p>0.05). At depths of 50-60 μm, only group L (KHN:17.05±1.29/18.26±1.30) differed statistically from the control (KHN:13.43±1.24/13.81±1.25), but not from the other groups. In conclusion, CO₂ laser alone was able to inhibit caries progression in the deepest layers. However, no synergistic effect was obtained when CO₂ laser irradiation and FFA application and were combined.

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Thaís M. Parisotto, Patrícia A. Sacramento, Marcelo C. Alves, Regina M. Puppin-Rontani, Maria Beatriz D. Gavião, and Marinês Nobre-dos-Santos "In vitro study of the effect of a pulsed 10.6μm CO₂ laser and fluoride on the reduction of carious lesions progression in bovine root dentin", Proc. SPIE 7549, Lasers in Dentistry XVI, 75490J (5 March 2010); https://doi.org/10.1117/12.842850

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KEYWORDS

Carbon dioxide lasers

Dental caries

In vitro testing

Laser irradiation

Minerals

Carbon monoxide

Fluorine

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