Polymer microspheres carrying fluorescent DNA probes

Xiaoyu Chen; Zhao Dai; Jimei Zhang; Shichao Xu; Chunrong Wu; Guo Zheng

doi:10.1117/12.869654

22 July 2010 Polymer microspheres carrying fluorescent DNA probes

Xiaoyu Chen, Zhao Dai, Jimei Zhang, Shichao Xu, Chunrong Wu, Guo Zheng

Proceedings Volume 7749, 2010 International Conference on Display and Photonics; 77490J (2010) https://doi.org/10.1117/12.869654
Event: 2010 International Conference on Display and Photonics, 2010, Nanjing, China

Abstract

A polymer microspheres carried DNA probe, which was based on resonance energy transfer, was presented in this paper when CdTe quantum dots(QDs) were as energy donors, Au nanoparticles were as energy accepters and poly(4- vinylpyrindine-co-ethylene glycol dimethacrylate) microspheres were as carriers. Polymer microspheres with functional group on surfaces were prepared by distillation-precipitation polymerization when ethylene glycol dimethacrylate was as crosslinker in acetonitrile. CdTe QDs were prepared when 3-mercaptopropionic acid(MPA) was as the stabilizer in aqueous solution. Because of the hydrogen-bonding between the carboxyl groups of MPA on QDs and the pyrindine groups on the microspheres, the QDs were self-assembled onto the surfaces of microspheres. Then, the other parts of DNA probe were finished according to the classic method. The DNA detection results indicated that this novel fluorescent DNA probe system could recognize the existence of complementary target DNA or not.

Citation Download Citation

Xiaoyu Chen, Zhao Dai, Jimei Zhang, Shichao Xu, Chunrong Wu, and Guo Zheng "Polymer microspheres carrying fluorescent DNA probes", Proc. SPIE 7749, 2010 International Conference on Display and Photonics, 77490J (22 July 2010); https://doi.org/10.1117/12.869654

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