Thermal and non-thermal fluctuations of the mechanical properties in living cells

Ming-Tzo Wei; H. Daniel Ou-Yang

doi:10.1117/12.860697

27 August 2010 Thermal and non-thermal intracellular mechanical fluctuations of living cells

Ming-Tzo Wei, H. Daniel Ou-Yang

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77621L (2010) https://doi.org/10.1117/12.860697
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Intracellular stresses generated by molecular motors can actively modify cytoskeletal network, which causes changes in intracellular mechanical properties. We study the out-of-equilibrium microrheology in living cells. This paper reports measurements of the intracellular mechanical properties using passive and optical tweezers-based active microrheology approaches and endogenous organelle particles as probes. Using the fluctuation-dissipation theorem, we compared the two approaches measurements and distinguished thermal and non-thermal fluctuations of mechanical properties in living cells.

Citation Download Citation

Ming-Tzo Wei and H. Daniel Ou-Yang "Thermal and non-thermal intracellular mechanical fluctuations of living cells", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621L (27 August 2010); https://doi.org/10.1117/12.860697

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