Reduction of noise floor for molecular, fluorescence-enhanced optical imaging

Banghe Zhu; John C. Rasmussen; Yujie Lu; Eva M. Sevick-Muraca

doi:10.1117/12.874331

22 February 2011 Reduction of noise floor for molecular, fluorescence-enhanced optical imaging

Banghe Zhu, John C. Rasmussen, Yujie Lu, Eva M. Sevick-Muraca

Proceedings Volume 7891, Design and Quality for Biomedical Technologies IV; 789104 (2011) https://doi.org/10.1117/12.874331
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Near infrared fluorescence (NIRF) optical imaging has been successfully demonstrated to offer a high specificity and sensitivity in detecting various diseases. However, the measurement sensitivity of NIRF optical imaging system is limited by strong backscattered excitation light leakage. Herein, appropriate filter combination and collimation optics was adapted to the NIRF optical imager. The sensitivity of near-infrared fluorescence imaging instrumentation can be dramatically improved upon using the appropriate filter combination and collimation optics. This validation and qualification approach to reduce the noise floor and improve sensitivity is presented a standardized metric for all fluorescence based imaging systems proposed.

Citation Download Citation

Banghe Zhu, John C. Rasmussen, Yujie Lu, and Eva M. Sevick-Muraca "Reduction of noise floor for molecular, fluorescence-enhanced optical imaging", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 789104 (22 February 2011); https://doi.org/10.1117/12.874331

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