Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence

Rajatesh Gudibande; Meghdoot Mozumder; Rajbeer Singh; Prasanta K. Panigrahi; Sharad Gupta; Asima Pradhan

doi:10.1117/12.875246

22 February 2011 Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence

Rajatesh Gudibande, Meghdoot Mozumder, Rajbeer Singh, Prasanta K. Panigrahi, Sharad Gupta, Asima Pradhan

Author Affiliations +

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 790220 (2011) https://doi.org/10.1117/12.875246
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Wavelet Transform based multi-resolution analysis has been used to characterize the intrinsic fluorescence of both dysplastic and normal human cervical tissues. The fluorescence spectra corresponding to 325nm and 370nm excitation from cervical dysplastic tissues of 48 patients from diverse age groups are studied in detail using Morlet wavelet. The wavelet modulus maxima lines for 325nm excitation indicated a distinct shift for dysplastic tissues towards the lower wavelengths with respect to normal ones. For 370nm excitation however, the shift for dysplastic tissues were towards the higher wavelengths. Sensitivities of 72% and 81% for spectral shifts of 325 and 370nm excitations were observed in the wavelength band of 425-500nm of the intensity spectra.

Citation Download Citation

Rajatesh Gudibande, Meghdoot Mozumder, Rajbeer Singh, Prasanta K. Panigrahi, Sharad Gupta, and Asima Pradhan "Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790220 (22 February 2011); https://doi.org/10.1117/12.875246

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available