Imaging amylopectin's order in starch using 3-dimensional polarization SHG

Sotiris Psilodimitrakopoulos; Ivan Amat-Roldan; David Artigas; Pablo Loza-Alvarez

doi:10.1117/12.889562

8 June 2011 Imaging amylopectin's order in starch using 3-dimensional polarization SHG

Sotiris Psilodimitrakopoulos, Ivan Amat-Roldan, David Artigas, Pablo Loza-Alvarez

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80860M (2011) https://doi.org/10.1117/12.889562
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and a methodology, which extracts molecular information below the experimental resolution limit. Firstly, it provides the pitch angle (SHG effective orientation) of the SHG source helix of the sample. This information is used to characterize and categorize the SHG sources among them. And secondly, it provides the degree of organization of the SHG source molecules. This can be used as a quantitative imaging biomarker able to characterize the degree of organization (homeostasis) of the sample. Here we applied the model in dried and hydrated wheat starch granules. Our results show that the SHG source molecule in starch is amylopectin. We also conclude that under hydration, the amylopectin molecules are further organized but they do not change structure. This organization is reflected to the width of the pitch angles pixels' histograms' distributions. The shorter the width is, the more organized the amylopectin molecules in starch are.

Citation Download Citation

Sotiris Psilodimitrakopoulos, Ivan Amat-Roldan, David Artigas, and Pablo Loza-Alvarez "Imaging amylopectin's order in starch using 3-dimensional polarization SHG", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80860M (8 June 2011); https://doi.org/10.1117/12.889562

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available