Angular resolved scattering microscopy

Thomas Rothe; Michael Schmitz; Alwin Kienle

doi:10.1117/12.889625

8 June 2011 Angular resolved scattering microscopy

Thomas Rothe, Michael Schmitz, Alwin Kienle

Proceedings Volume 8086, Advanced Microscopy Techniques II; 808613 (2011) https://doi.org/10.1117/12.889625
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

The analysis of particle size and structure without any manipulation by exogenous markers is essential for investigating biological cells and tissues. For this purpose we built up a scattering microscope and already proofed that spectral resolved scattering microscopy is suitable to detect differences in sphere diameters of a few nanometers. Using an angular resolved scattering microscope permits to distinguish diameters of single polystyrene spheres with a standard deviation of less than 1 %. The setup consists of an inverse microscope with a reflected darkfield illumination that is realized by a collimated beam with a well-defined angle. A supercontinuum laser in combination with an acousto-optic tunable filter allows wavelength tuning of free choice. For validation we measured single polystyrene beads at different wavelengths and determined the diameters by correlating measurements of the scattered light with the theoretical angular distribution based on Mie theory. The average diameter of a single polystyrene bead was determined with a relative standard deviation of 0.65%.

Citation Download Citation

Thomas Rothe, Michael Schmitz, and Alwin Kienle "Angular resolved scattering microscopy", Proc. SPIE 8086, Advanced Microscopy Techniques II, 808613 (8 June 2011); https://doi.org/10.1117/12.889625

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available